|
|
|
| Registros recuperados: 233 | |
|
|
| Duarte,E.R.; Hamdan,J.S.. |
| A molecular study of Malassezia strains isolated from cattle with or without otitis was carried out by random amplified polymorphic DNA analysis (RAPD). DNA was extracted and purified from nine strains of Malassezia sympodialis and fourteen of Malassezia furfur. These microorganisms were collected from eight different bovine herds in Minas Gerais state, Brazil. The RAPD analysis and phenograms did not show the formation of genetically distinct groups among the strain isolated from cattle with or without otitis raised in the same herds. Genetic heterogeneity was observed among Malassezia strains from different geographic origins. These data suggest that genetically similar M. sympodialis and M. furfur strains found as members of the normal ear microbiota... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine; Otitis; Malassezia spp.; RAPD. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000400001 |
| |
|
| |
|
|
| Chitarra,Cristiane Silva; Silva,Mayara Inácio Vincenzi da; Brandão,Laila Natasha Santos; Kagueyama,Francielle Cristina; Candido,Stefhano Luis; Rosa,Janaina Marcela Assunção; Nakazato,Luciano; Dutra,Valéria. |
| ABSTRACT: Swine respiratory diseases such as atrophic rhinitis and bronchopneumonia caused by Pasteurella (P.) multocida cause important economic losses to the modern swine industry. The purpose of this study was to characterize P. multocida strains isolated from swine lungs by RAPD (Randomly Amplified Polymorphic DNA) to demonstrate their genetic diversity. Ninety-four samples of fragments from lungs with pneumonia and sixty one samples without pneumonia were collected in slaughterhouses in Mato Grosso during the period from December 2009 to March 2010. Clinical cases in 2012 and 2013 were also included in this study. Among the lung fragments with macroscopic lesions, without macroscopic lesions and clinical samples, 40.42%, 4.49% and 100% were positive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pulmonary pasteurellosis; Swine; Pneumonia; Pasteurella multocida; RAPD. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000100119 |
| |
|
| |
|
|
| Cansian, Rogério Luis; URI; Mossi, Altemir José; URI; Di-Luccio, Marco; URI; Cechet, Michel Luis; URI; Mazutti, Marcio; URI; Echeverrigaray, Sergio; UCS. |
| A progeny of Cambona-4, a female maté plant (Ilex paraguariensis) selected by its agronomical characteristics and mild flavor, was evaluated using RAPD markers in order to identify its male parents. Using RAPD markers specifics of each one of the four potential males, the paternity of 84 out of 107 offsprings was confirmed. The majority of the offsprings (83.3%) were ascribed to pollen donor A, while male plants B, C, and D represented 11.9, 4.8, and 0%, respectively. The highly desirable agronomical characteristics and product quality of Cambona-4/pollen donor A offsprings, identified by RAPD markers, lead to the planting of an orchard Cambona-4 and pollinator A to obtain bi-clonal commercial seeds. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ilex paraguariensis; Mate; RAPD; Paternity 2.03.03.02-5. |
| Ano: 2009 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/764 |
| |
|
|
| Dizbay,Murat; Kalkanci,Ayse; Sezer,Busra Ergut; Aktas,Firdevs; Aydogan,Sibel; Fidan,Isil; Kustimur,Semra; Sugita,Takashi. |
| We investigated a nosocomial cluster of four Candida parapsilosis fungemia episodes that occurred in a neurological intensive care unit over a two-week period. The four infected patients had received parenteral nutrition through central lines, and all four had catheter-related candidemia. All of the isolates were susceptible to all of the antifungals tested, including amphotericin B, fluconazole, voriconazole, and caspofungin. They had strictly related fingerprints, based on randomly amplified polymorphic DNA analysis. Additional DNA sequencing data revealed that they were same strain. Although no isolate of Candida parapsilosis was recovered from other clinical, surveillance, or environmental samples, nosocomial spread of this yeast ceased, following the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candidemia; Candida parapsilosis; Nosocomial outbreak; Intensive care unit; RAPD; DNA sequencing. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000500010 |
| |
|
| |
|
|
| Povh,J.A.; Ribeiro,R.P.; Lopera-Barrero,N.M.; Gomes,P.C.; Blanck,D.V.; Vargas,L.; Jacometo,C.B.; Lopes,T.S.. |
| Avaliou-se a variabilidade genética dos estoques de reprodutores e dos peixes jovens de Piaractus mesopotamicus de três pisciculturas do estado do Paraná, utilizadas no programa de aumento de estoque de peixes no rio Paranapanema. Foi utilizado o marcador RAPD para avaliar as amostras do estoque de reprodutores e dos peixes jovens das pisciculturas de Palotina, Cambará e Andirá. A porcentagem de fragmentos polimórficos e o índice de diversidade genética de Shannon dos estoques de reprodutores variaram de 75,0% a 71,4% e de 0,434 a 0,376, respectivamente. Os peixes jovens das pisciculturas apresentaram valores mais elevados para ambos os parâmetros, com exceção da piscicultura de Palotina, na qual o índice de diversidade genética de Shannon foi semelhante.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Piaractus mesopotamicus; Peixes jovens; Estoque de reprodutores; RAPD; Variabilidade genética. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000500023 |
| |
|
|
| Povh,Jayme A; Lopera Barrero,Nelson M; Ribeiro,Ricardo P; Lupchinski Jr.,Enio; Gomes,Patricia C; Lopes,Tais S. |
| Las poblaciones de peces nativos están disminuyendo debido a varios factores que impactan en el ambiente. Como forma de abordar este problema, se han realizado varias acciones como por, ejemplo, el repoblamiento. Sin embargo, estas acciones se realizan sin ningún respaldo científico. Esto puede, además de no ser eficiente, provocar una pérdida de la diversidad genética en las poblaciones, pudiendo ocasionar impactos irreversibles en las próximas generaciones. Por esta razón, el empleo de marcadores moleculares, que permitan monitorear genéticamente las poblaciones parece fundamental para lograr la conservación de la ictiofauna |
| Tipo: Journal article |
Palavras-chave: Conservación genética; Ictiofauna; Microsatélite; Pez; RAPD; Variabilidad genética. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202008000100001 |
| |
|
|
| Ibarra Estrada, Martha Elva. |
| A México se le considera centro de origen del zapote mamey ya que cuenta con una gran diversidad. Sin embargo, son pocos los trabajos enfocados en estudiar tal diversidad tanto en la parte morfológica, contenido de ácidos graso o aspectos moleculares. Por lo anterior, el objetivo del presente trabajo fue: caracterizar morfológica, bioquímica y molecularmente selecciones sobresalientes de zapote mamey. En base a las variables de fruto y el perfil de ácidos grasos en semilla, el material vegetal estudiado mostró amplia variación entre las accesiones. El peso y longitud de fruto, longitud de hilio y de semilla, número de fibras, grosor de epicarpio y ancho de semilla fueron las variables determinantes para la agrupación de las selecciones. El perfil de... |
|
Palavras-chave: Caracterización; Frutos; Variabilidad; RAPD; RAMP; Characterization; Fruits; Lipids; Seeds; Variability; Lípidos; Semillas; Doctorado; Fruticultura. |
| Ano: 2012 |
URL: http://hdl.handle.net/10521/740 |
| |
|
|
| Pereira,Márcio; Oliveira,Antonio Luis de; Pereira,Regina Eli de Almeida; Sena,Janete Apparecida Desidério; Costa,Juliana Regina Vieira da; Almeida,Marcílio de; Gonçalves,Antonio Natal. |
| The jabuticaba tree is considered one of the most typical Brazilian fruit trees. However, few studies of this plant are found in the literature and even its botanical classification is very controversial. The present research reports some comparisons between jabuticaba species, using morphologic (organography) and molecular markers techniques. The morphologic characteristics of the plant used as markers were compared with specimens of the herbaria from São Paulo and Minas Gerais States and with the descriptions reported in the literature. Molecular differences between the species were identified by the use of RAPD markers. The experiment was made in Piracicaba, Jaboticabal and Ituverava municipal districts in São Paulo State, Brazil. Morphologic and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Jabuticaba trees; Biological identification; Morphologic markers; Molecular markers; RAPD. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452005000300040 |
| |
|
| |
|
|
| Romanelli,RMC; Clemente,WT; Lima,SSS; Rezende,EM; Martinho,GH; Paiva,LFR; Neves,FAC; Madeira,JGC; Amâncio,GCS; Lima,AS; Faria,LC; Coutinho,RL. |
| Methicillin-resistant Staphylococcus aureus (MRSA) infections frequently complicate the post-operative course of transplant recipients, and despite nasal carriage and endemic colonization, MRSA outbreaks are not commonly described. This study reports a case of MRSA outbreak and discusses infection control measures and recommendations for this situation. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: MRSA; Outbreak; Surgical site infection; Liver transplantation; RAPD. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000100011 |
| |
|
| |
|
|
| Almeida,Álvaro M. R.; Piuga,Fernanda F.; Marin,Silvana R. R.; Binneck,Eliseu; Sartori,Fábio; Costamilan,Leila M.; Teixeira,Maria R. O.; Lopes,Marcelo. |
| Cercospora kikuchii, involved with the defoliation of soybean (Glycine max) plants, is normally associated with Septoria glycines in late season. Seventy-two isolates from different regions in Brazil, obtained mainly from purple stained seeds, showed phenotypic variation. Cercosporin content and rate of colony growth was higly variable among isolates. A strong correlation between cercosporin content and virulence was identified. Genetic variation among and within populations was evaluated based on 86 RAPD loci. The RAPD analysis clustered all isolates into seven groups. No relationship was observed between RAPD groups and geographic origin or cercosporin content. The sequence of the internal spacer regions (ITS1-5.8S-ITS2) from 13 isolates chosen according... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genotypic diversity; PCR-RFLP; RAPD; ITS sequence; Virulence. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000600005 |
| |
|
|
| Robe,Lizandra Jaqueline; Silva,Luciano Basso da; Loreto,Elgion Lucio da Silva. |
| Traditionally, the Drosophila guarani species group has been divided into two subgroups: the guarani and the guaramunu subgroups. Two, out of the four species included in this research, are members of the guarani subgroup (D. ornatifrons Duda, 1927 and D. subbadia Paterson & Mainland, 1943) and two are included in the guaramunu subgroup (D. maculifrons Duda, 1927 and D. griseolineata Duda, 1927). However, some authors have suggested that D. maculifrons and D. griseolineata are much closer to some species of the Drosophila tripunctata group than to some of the species of the guarani group. To add new data to the matter under dispute, Polyacrylamide Gel Eletrophoresis (PAGE-SDS) was used for the analysis and comparison of protein composition and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Drosophila; Guarani group; Monophyly; Phylogeny; RAPD. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262002000400004 |
| |
|
|
| Dergam,Jorge A.; Paiva,Samuel R.; Schaeffer,Carlos E.; Godinho,Alexandre L.; Vieira,Fabio. |
| In the Rio Doce basin of southeastern Brazil, the freshwater fish Hoplias malabaricus (trahira) is a widespread predatory characin and one of the few resilient native fishes in a highly impacted lake system. In order to test for genetic differentiation in populations within this basin and for biogeographic relationships among populations of this species in other basins, a study was conducted using RAPD-PCR analysis of Rio Doce samples (N = 63) and phylogeographic analyses with mitochondrial DNA (mtDNA) haplotypes, including the Rio Grande and Macacu river basins. In the Rio Doce basin, the patterns of genetic similarity of RAPD-PCR markers (individual fingerprinting and Neis genetic distance) suggest the existence of two genetically different groups, one... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RAPD; SSCP; MtDNA; Phylogeography; Hoplias malabaricus; Conservation genetics. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400005 |
| |
|
| |
|
|
| Panarari, Renata de Souza; UEM; Prioli, Alberto José; UEM; Prioli, Sonia Maria Alves Pinto; UEM; Souza, Maria Conceição de; NUPELIA; Oliveira, Alessandra Valéria de; UEM; Boni, Talge Aiex; UEM; Lucio, Léia Carolina; UEM; Prioli, Leudenir Maria; UEM. |
| No Sul e Sudeste do Brasil, Lonchocarpus cultratus (Vell.) A.M.G. Azevedo e H.C. Lima é uma espécie arbórea importante para o reflorestamento natural, incluindo áreas ripárias. Em florestas regeneradas, diferentemente de florestas não perturbadas, esta espécie tem sido observada como agregados de plantas semelhantes entre si, ao ponto de ter sido formulada a hipótese de serem clones. Em um estudo prévio, brotamento de raízes foi observado em L. cultratus, em uma floresta afetada pelo fogo. No presente estudo, análises de polimorfismo de RAPD revelaram alta diversidade genética entre as plantas de agregados de L. cultratus, em uma floresta ripária afetada pelo fogo, no alto rio Paraná. Os dados permitem sugerir que reprodução sexual tem sido a usual... |
|
Palavras-chave: 2.01.00.00-0 Biologia Geral Lonchocarpus; Rio Paraná; RAPD; Reflorestamento 2.01.00.00-0 Biologia Geral. |
| Ano: 2004 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/1596 |
| |
|
| |
| Registros recuperados: 233 | |
|
|
|
